Ultra-high multiplex analytical systems and methods

G - Physics – 02 – B

Patent

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G02B 6/12 (2006.01) G01N 21/00 (2006.01)

Patent

CA 2738626

Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

La présente invention concerne un appareil, des systèmes et des procédés permettant d'analyser des réactions discrètes en multiplexage ultra-élevé présentant un bruit optique réduit et une souplesse accrue du système. L'appareil comprend des substrats comportant des composants optiques intégrés qui augmentent la capacité de multiplexage d'une ou de plusieurs régions de réaction de densité croissante, améliorant la transmission de la lumière vers les régions de réactions discrètes ou la collecte de lumière à partir de ces régions. Les composants optiques intégrés comprennent des éléments optiques réfléchissants qui redirigent la lumière d'éclairage et la lumière émise à partir des régions discrètes afin de collecter plus efficacement la lumière émise. Les applications particulièrement préférées comprennent une analyse de réaction à molécule unique, telle que la synthèse d'un acide nucléique dépendant d'un modèle et à médiation par polymérase et la détermination de séquences.

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