Ultrahigh copy number streptomycetes plasmids

Details Ultrahigh copy number streptomycetes plasmids Ultrahigh copy number streptomycetes plasmids

C - Chemistry, Metallurgy

12

N

195/1.18, 195/1.

C12N 1/21 (2006.01) C12N 15/69 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1281670

X-6786A Abstract The present invention discloses an ~1.4 kb BclI-Sau3A restriction fragment containing the minimal replicon of the Streptomyces coelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid deriva- tives constructed by in vitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyces lividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.

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