Ultrasensitive detection of pathogenic microbes

Details Ultrasensitive detection of pathogenic microbes Ultrasensitive detection of pathogenic microbes

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C07H 21/00 (2006.01) C12N 15/30 (2006.01) C12P 19/34 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/18 (2006.01)

Patent

CA 2448098

The present invention describes a 5' nuclease real-time polymerase chain reaction (PCR) approach for the quantification of total coliforms, E. coli, toxigenic E. coli O157:H7, toxigenic M. aeruginosa (microcystin hepatotoxins), Giardia lamblia, and Cryptosporidium parvum, based on the specific identified primer and probe sequences from the lacZ (E. coli), eaeA (E. coli O157:H7), mcyA (M aeruginosa), .beta.- giardin (G. lamblia), and COWP (C. parvum) genes respectively. The invention allows for the detection of all of the aforementioned microbes, with or without culture enrichments, utilizing a 5' nuclease PCR approach. The invention also provides primer and probe sequences useful to produce detectable amplicons, by any amplification method, which are diagnostic for such organisms.

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