Uncoupled productive and catabolic host cell pathways

Details Uncoupled productive and catabolic host cell pathways Uncoupled productive and catabolic host cell pathways

C - Chemistry, Metallurgy

12

P

C12P 25/00 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 15/52 (2006.01) C12N 15/54 (2006.01) C12N 15/63 (2006.01) C12P 1/00 (2006.01) C12P 7/60 (2006.01) C12P 19/02 (2006.01) C12P 19/30 (2006.01)

Patent

CA 2443145

The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.

L'invention porte sur des procédés de production de produits comprenant des cellules hôtes améliorées génétiquement modifiées de sorte qu'elles aient des mécanismes d'action productifs et cataboliques non couplés. Cette invention porte notamment sur des cellules hôtes ayant une modification dans l'acide nucléique codant une activité enzymatique endogène qui phosphoryle D-glucose au niveau de son 6ème carbone et/ou une modification de l'acide nucléique codant une activité enzymatique qui phosphoryle D-gluconate au niveau de 6ème carbone. Ces cellules hôtes améliorées sont utilisées dans la produciton de produits tels que es intermédiaires de l'acide ascorbique. L'invention porte également sur des procédés de fabrication et d'utilisation des cellules hôtes améliorées, et sur des séquences d'acide nucléiques et d'acides aminés pour la glucokinase et la gluconokinase.

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