Ungulate eg cell

Details Ungulate eg cell Ungulate eg cell

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01K 67/027 (2006.01) C12N 5/06 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2192982

A porcine EG cell line has been established which is derived from porcine primordial germ cells, has a multipotency or totipotency like that of an embryonic stem cell, and can be subcultured on feeder cells. The line is reactive with a monoclonal antibody 4C9 prepared by using a mouse embryonic carcinoma cell F9 strain as the antigen. As the established EG cell line can be readily subjected to gene manipulation under culture, in particular, gene targeting, it is easy to produce a variety of swine by gene manipulation.

Une souche cellulaire EG d'origine porcine a été isolée à partir de cellules reproductrices primordiales d'origine porcine. Cette souche, qui présente une multipotence ou une totipotence identique à celle d'une cellule souche d'embryon, peut faire l'objet d'un repiquage sur des cellules nourricières. La souche réagit à un anticorps monoclonal 4C9 préparé à l'aide d'une souche de cellule F9 de carcinome embryonnaire de souris faisant office d'antigène. Sous culture, cette souche cellulaire EG isolée peut être facilement soumise à une manipulation génétique, et notamment à un ciblage génique, ce qui permet l'obtention facile d'une variété porcine par manipulation génétique.

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