Universal arrays

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2366459

An array of oligonucleotides on a solid substrate is disclosed, which can be used for multiple purposes. Methods and reagents are provided for performing genotyping to determine the identity or ration of allelic forms of a gene in a sample. A single base extension primer is coupled to a sequence identity code. During the primer extension reaction a distinctive label is incorporated which identifies the allelic form present in the sample. This permits multiple simultaneous analyses to be performed easily and efficiently.

L'invention concerne un réseau d'oligonucléotides sur un substrat solide pouvant servir à de multiples fins. Des procédés et réactifs permettent d'effectuer du génotypage pour déterminer l'identité ou le taux de formes alléliques d'un gène dans un échantillon. Une amorce d'extension de base simple est couplée à un code d'identité de séquence. Lors de la réaction d'extension d'amorce, une étiquette distinctive est intégrée et permet d'identifier la forme allélique présente dans l'échantillon. On peut ainsi effectuer aisément et d'une manière efficace de nombreuses analyses simultanées.

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