Universal biological indicator system

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

21/16, 150/16.2

C12Q 1/22 (2006.01) A61L 2/26 (2006.01) C12M 1/28 (2006.01)

Patent

CA 2010063

A cylindrical, transparent capsule (10) is less than half filled with a liquid culture medium (12). An open end of the capsule is closed by supporting annulus (24) and a vent tube (28) having a vent aperture (30) at a free end thereof. The vent aperture is disposed at a volumetric center of the capsule such that an upper surface of the liquid culture medium is disposed below the vent aperture in any orientation of the capsule. A heat labile membrane (32) which becomes porous in the presence of steam seals the vent passage but provides a pressure escape from the capsule at steam sterilizinq temperatures. A disk inoculated with microorganisms is supported by the annular support. A cap (40) is slidably movable between a first position in which apertures (42) provide a liquid flow path between the inoculated disk and the exterior and a second position in which a severing ring (46) severs the support annulus bringing the inoculated disk and the culture medium into contact.

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