Universal chloroplast integration and expression vectors,...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2299609

The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants are transformed which have never been transformed heretofore. Plants transformed with a synthetic gene express valuable biodegradable protein-based polymers (PBPs). Transformed plants produce high value molecules. Resistance is provided to agricultural crops against the major classes of chemical herbicides. Herbicide resistance is used as a lethal selectable marker for chloroplast transformation. The transformed plants are capable of expressing in addition to the targeted trait, a desirable, secondary non-targeted trait. Insect resistance is provided to transformed plants, both against insects that are susceptible to Bt toxins and against insects that have developed resistance to Bt toxins.

L'invention porte sur des vecteurs universels d'intégration et d'expression de chloroplastes aptes à transformer et intégrer de manière stable des gènes dans le génome chloroplastique de plusieurs espèces de plantes. L'invention porte également sur des plantes transformées et leur descendance. On transforme les monocotylédones et les dicotylédones qui n'ont jamais été transformées auparavant. Les plantes transformées par un gène synthétique expriment des polymères à base de protéines biodégradables utiles. Les plantes transformées produisent des molécules de grande valeur. Les récoltes agricoles bénéficient ainsi d'une résistance aux principales classes d'herbicides chimiques. On utilise la résistance aux herbicides comme marqueur sélectionnable létal dans la transformation chloroplastique. Les plantes transformées peuvent exprimer non seulement un caractère ciblé, mais aussi un caractère non ciblé, secondaire, souhaitable. On confère également aux plantes transformées une résistance aux insectes, à la fois aux insectes sensibles aux toxines Bt et aux insectes ayant développé une résistance à ces toxines.

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