Universal method and composition for the rapid lysis of...

Details Universal method and composition for the rapid lysis of... Universal method and composition for the rapid lysis of...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2451985

This invention describes a rapid (10 to 15 minutes), simple, flexible and efficient method of nucleic acids extraction for nucleic acid testing assays. This method has the following basic steps: i) mechanical cell lysis using solid particles in the presence of a chelating agent, followed by ii) controlling the presence and/or activity of NAT assays inhibitors. This method is applicable to various biological samples and universal for microorganisms, as one can use it to extract nucleic acids from test samples containing target viruses, bacteria, bacterial spores, fungi, parasites or other eukaryotic cells, including animal and human cells.

L'invention concerne un procédé rapide (10 à 15 minutes), simple, flexible et efficace d'extraction d'acides nucléiques pour des tests faisant intervenir des acides nucléiques (NAT). Ce procédé comprend principalement les étapes suivantes : i) lyse mécanique de cellules au moyen de particules solides en présence d'un agent chélateur et ii) contrôle de la présence et/ou de l'activité d'inhibiteurs de tests NAT. Ce procédé peut être appliqué à divers échantillons biologiques et il est universel pour les microorganismes, ledit procédé pouvant être mis en oeuvre pour extraire des acides nucléiques d'échantillons contenant des microorganismes cibles tels que des virus, des bactéries, des spores bactériennes, des champignons, des parasites ou des cellules eucaryotes, notamment des cellules animales et humaines.

Affiliated with

Also associated with

Rating

Universal method and composition for the rapid lysis of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Universal method and composition for the rapid lysis of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Universal method and composition for the rapid lysis of... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1741111