Universal primer sequence for multiplex dna amplification

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2223729

The present invention provides primers that allow simultaneous amplification of multiple DNA target sequences present in a DNA sample. Further provided are methods for detecting multiple defined target DNA sequences in a DNA sample. Methods for high-throughput genetic screening are also provided. In yet another aspect, the present invention provides single-stranded oligonucleotide DNA primers for amplification of a target DNA sequence in a multiplex polymerase chain reaction.

L'invention concerne des amorces permettant une amplification simultanée de séquences cibles multiples d'ADN présentes dans un échantillon d'ADN, des procédés pour détecter des séquences cibles multiples définies d'ADN dans un échantillon d'ADN, des procédés de criblage génétique à haute capacité de traitement, ainsi que des amorces constituées d'oligonucléotides d'ADN à un seul brin pour l'amplification d'une séquence d'ADN cible dans une PCR multiplex.

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