C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2320575
Signal primers are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The signal primer comprises a first and a second oligonucleotide and is partially single-stranded and partially double- stranded. In the presence of target, the. second oligonucleotide of the signal primer. is displaced from the first and a conformational change in a reporter probe occurs which changes the distance between the members of a donor/quencher dye pair linked to the reporter probe. The change in proximity between the dyes causes an increase or a decrease in fluorescence quenching, which is detected as an indication of the presence of the target sequence.
Dean Cheryl H.
Linn C. Preston
Nadeau James G.
Pitner J. Bruce
Walker G. Terrance
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
LandOfFree
Universal probes and methods for detection of nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Universal probes and methods for detection of nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Universal probes and methods for detection of nucleic acids will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2011969