Universal rinse reagent composition for use in hematological...

G - Physics – 01 – N

Patent

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G01N 33/49 (2006.01) A61L 2/18 (2006.01) B08B 3/08 (2006.01) C11D 1/72 (2006.01) C11D 1/722 (2006.01) C11D 3/04 (2006.01) C11D 3/37 (2006.01) C11D 3/48 (2006.01) C12Q 1/06 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2174306

The present invention provides a heretofore unknown use for a aqueous reagent composition that serves as a universal rinse for performing and/or improving a variety of hematological analyses on automated analyzers. The universal rinse reagent comprises a phosphate buffer to maintain the rinse solution pH at from about 7.0 to about 7.6; a nonhemolytic nonionic surfactant, such as a Pluronic; an alkali metal salt, such as NaCl; antimicrobial and anti-oxidant compounds; and has an osmolality of about 285 to 305 mOsmol/kg. The universal rinse reagent composition is highly suitable for use in the rinse phases or cycles of all types of blood cell analysis methods and processes performed on semi- and fully-automated systems. The invention allows the replacement of mutiple and specific rinse solutions with the disclosed universal rinse to obtain accurate and acceptable results, independent of the types of blood cell analyses that are performed. The universal rinse is most particularly useful for automated systems having intricate hardware and a number of different input and output channels. The universal rinse composition serves to economize, streamline, and simplify the design and operation of such systems.

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