Universal solid-phase hybridization apparatus

Details Universal solid-phase hybridization apparatus Universal solid-phase hybridization apparatus

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2282296

A method of determining the presence of a nucleic acid analyte from a sample comprising binding a biotinylated capture oligonucleotide to a solid phase non- covalently coated with streptavidin or an avidin derivative. The capture oligonucleotide is contacted with and selectively hybridized to, under stringent hybridization conditions, a nucleic acid analyte to form a detectable hybridization complex.

La présente invention concerne un procédé de détermination de la présence d'un analyte d'acide nucléique à partir d'un échantillon, consistant à lier un oligonucléotide de capture biotinylé à une phase solide enrobée par liaison non covalente d'une streptavidine ou d'un dérivé d'avidine. L'oligonucléotide de capture est mis en contact avec un analyte d'acide nucléique et sélectivement hybridé au dit acide nucléique, dans des conditions d'hybridation rigoureuses, de façon qu'un complexe d'hybridation détectable soit formé.

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