Unnatural reactive amino acid genetic code additions

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) C07C 229/36 (2006.01) C07K 16/40 (2006.01) C08G 73/00 (2006.01) C09B 56/00 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 11/00 (2006.01)

Patent

CA 2527877

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsynthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

L'invention concerne des compositions et des procédés de production de composants transactionnels qui augmentent le nombre d'aminoacides codés génétiquement dans des cellules eucaryotes. Lesdits composants comprennent des ARNt orthogonaux, des synthétases d'ARNt-aminoacyle orthogonal, des paires orthogonales d'ARNt/de synthétases d'ARNt et des aminoacides artificiels. L'invention concerne également des protéines et de procédés permettant de produire des protéines avec des aminoacides dans des cellules eucaryotes.

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