Upflow settling reactor for enzymatic hydrolysis of cellulose

C - Chemistry – Metallurgy – 12 – P

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Details

C12P 19/14 (2006.01) C12P 7/10 (2006.01) C12P 19/02 (2006.01) C12P 19/12 (2006.01)

Patent

CA 2590972

A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre~treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and maintaining an average slurry flow velocity of about 0.1 to about 20 feet per hour, such that the undissolved solids flow upward at a rate slower than that of the liquid. Cellulase enzymes are added to the aqueous slurry before or during the step of introducing. An aqueous stream comprising hydrolysis product and unhydrolyzed solids is removed from the hydrolysis reactor. Also provided are enzyme compositions which comprise cellulase enzymes and flocculents for use in the process. In addition, a kit comprising cellulase enzymes and flocculent is provided.

L'invention concerne un procédé d'hydrolyse enzymatique de cellulose permettant d'obtenir un produit d'hydrolyse à partir d'une charge d'alimentation cellulosique prétraitée. Ce procédé consiste à introduire une suspension épaisse aqueuse de la charge d'alimentation cellulosique prétraitée au fond d'un réacteur d'hydrolyse. Pour limiter la dispersion axiale dans le réacteur, on évite de mélanger et on maintient une vitesse moyenne d'écoulement de la suspension épaisse entre environ 0,1 et environ 20 pieds par heure, de sorte que les solides non dissous s'écoulent de manière ascendante à un débit moindre que celui du liquide. Des enzymes cellulase sont ajoutées à la suspension épaisse aqueuse avant ou pendant l'étape d'introduction de la suspension épaisse. Un flux aqueux comprenant le produit d'hydrolyse et les solides non hydrolysés est retiré du réacteur d'hydrolyse. L'invention concerne également des compositions enzymatiques qui comprennent des enzymes cellulase et des floculants destinés à être utilisés dans le processus. L'invention concerne également une trousse comprenant des enzymes cellulase et des floculants.

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