Upstream activator sequences and recombinant promoter...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 15/67 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2221468

The invention concerns an upstream activating sequence (UAS) functional in Yarrowia consisting essentially of at least one copy of a selected sequence, and a recombinant promoter sequence comprising such an UAS. It concerns vectors comprising the recombinant promoter sequence and recombinant yeasts containing them, as well as process for producing a protein in yeast.

Cette invention porte sur une séquence activatrice en amont (UAS), fonctionnelle dans la levure Yarrowia, consistant principalement en une copie, au moins, d'une séquence sélectionnée ainsi que sur une séquence promotrice de recombinaison comportant cet UAS. Elle concerne également des vecteurs comprenant la séquence promotrice de recombinaison ainsi que des levures de recombinaison les contenant et un procédé de production d'une protéine dans la levure.

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