C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/74 (2006.01)
Patent
CA 2595594
Methods of isolating clinical-grade plasmid DNA from manufacturing processes, including large-scale fermentation regimes, are disclosed which encompass alternatives to two core unit operations common to plasmid DNA purification processes. The novel upstream and downstream purification processes disclosed herein provide for reduced production costs and increase process robustness. Either or both of the purification processes disclosed herein may be used in combination with additional purification steps known in the art that are associated with DNA plasmid purification technology.
L'invention concerne des procédés destinés à isoler un ADN plasmidique de qualité clinique à partir de procédés de fabrication, y compris des régimes de fermentation à large échelle, et incluant des variantes aux deux opérations unitaires de base communes aux procédés de purification d'ADN. Les nouveaux procédés de purification amont et aval de l'invention permettent de réduire les coûts de production et d'augmenter la robustesse du procédé. L'un ou l'autre de ces procédés de purification ou les deux peuvent être utilisés en combinaison avec des étapes de purification supplémentaires connues dans la technique qui sont associées à la technologie de purification de plasmide d'ADN.
Boyd David B.
Kristopeit Adam Joel
Lander Russel Jackson
Murphy Jason C.
Winters Michael Albert
Merck & Co. Inc.
Merck Sharp & Dohme Corp.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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