Urine based detection of a disease state caused by a...

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G01N 33/487 (2006.01) G01N 24/08 (2006.01) G01N 30/02 (2006.01) G01N 30/62 (2006.01) G01N 33/569 (2006.01) G01R 33/465 (2006.01)

Patent

CA 2721129

There is provided a method of diagnosing a disease state in a subject. The disease state is caused or effected by pneumococcal infection. The method includes obtaining a biological test sample from the subject. The biological sample includes at least one metabolite selected from the group consisting of citrate, trigonelline, acetylcarnitine, acetone, myo-inositol, 3-hydroxybutyrate, glucose and carnitine. A respective concentration of each one of the at least one metabolite is compared with a predetermined concentration value associated with a corresponding one of the at least one metabolite. The predetermined concentration value is indicative of the disease state. For example, the biological sample includes any one of citrate, trigonelline, acetylcamitine, acetone, myoinositol, 3-hydroxybutyrate, glucose and carnitine. As a further example, the biological samples include any combination of citrate, trigonelline, acetylcamitme, acetone, myo-xnositol, 3- hydroxybutyrate, glucose and carnitine. As a further example, the biological sample includes each one of citrate, trigonelline, acetylcamitinc, acetone, myoinositol, 3-hydroxybutyrate, glucose and carnitine.

L'invention se rapporte à un procédé permettant de diagnostiquer un état pathologique chez un sujet. L'état pathologique est provoqué ou créé par une infection à pneumocoque. Le procédé inclut l'obtention d'un échantillon de test biologique de la part du sujet. L'échantillon biologique inclut au moins un métabolite choisi dans le groupe comprenant le citrate, la trigonelline, l'acétylcarnitine, l'acétone, le myo-inositol, le 3-hydroxybutyrate, le glucose et la carnitine. Une concentration respective d'un ou des métabolites est comparée à une valeur de concentration prédéterminée associée au(x) métabolite(s) correspondant(s). La valeur de concentration prédéterminée est symptomatique de l'état pathologique. Par exemple, l'échantillon biologique inclut l'un quelconque des métabolites parmi le citrate, la trigonelline, l'acétylcarnitine, l'acétone, le myo-inositol, le 3-hydroxybutyrate, le glucose et la carnitine. A titre d'exemple supplémentaire, les échantillons biologiques incluent n'importe quelle combinaison des métabolites parmi le citrate, la trigonelline, l'acétylcarnitine, l'acétone, le myo-inositol, le 3-hydroxybutyrate, le glucose et la carnitine. A titre d'exemple supplémentaire, l'échantillon biologique inclut chacun des métabolites parmi le citrate, la trigonelline, l'acétylcarnitine, l'acétone, le myo-inositol, le 3-hydroxybutyrate, le glucose et la carnitine.

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