Urokinase zymogen and composition containing the same

C - Chemistry – Metallurgy – 12 – N

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195/127, 195/39.

C12N 9/72 (2006.01) C07K 16/40 (2006.01)

Patent

CA 1258242

Abstract of the Disclosure There is provided a zymogen of plasminogen activator which is produced by culturing human kidney cells in a culturing medium and recovering from the culture fluid by affinity chromatography using a column of immobilized anti-zymogen antibody. The zymogen is a polypeptide having a single chain with a molecular weight of about 50,000. Although the zymogen itself little exhibits plasminogen activator activity, it mani- fests a significant plasminogen activator activity by catalytic amounts of plasmin. The zymogen has a throm- bolytic ability as high as three times that of a human urinary urokinase, and the zymogen is usable for treating human thrombus. The zymogen can be stabilized by the addition of albumin and thus its stable liophy- lized preparation can be prepared.

462860

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