C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/54 (2006.01) C12N 15/75 (2006.01)
Patent
CA 2703951
The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme in the relative absence of serine protease enzyme contaminants. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising Bacillus strains engineered to be deficient in multiple serine proteases, and their use in production of recombinant neutral metalloprotease(s).
La présente invention propose des procédés et des compositions comprenant au moins une enzyme de type métalloprotéase neutre en l'absence relative de contaminants enzymatiques de type sérine protéase. La métalloprotéase neutre peut être utilisée pour le nettoyage et dans d'autres applications. Dans un aspect particulièrement préféré, la présente invention propose des procédés et des compositions contenant des souches Bacillus génétiquement modifiées afin de supprimer la production des multiples sérines protéases, et leur utilisation pour produire une ou plusieurs métalloprotéase(s) neutre(s) recombinante(s).
Lee Edwin
Shaw Andrew
Van Kimmenade Anita
Wallace Louise
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Danisco Us Inc.
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