Use and production of storage-stable neutral metalloprotease

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/54 (2006.01)

Patent

CA 2624977

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

L'invention concerne des procédés et des compositions contenant au moins une enzyme de métalloprotéase neutre présentant une stabilité de stockage améliorée. Dans certains modes de réalisation, la métalloprotéase neutre s'utilise dans le nettoyage et dans d'autres applications. Dans certains modes de réalisation préférés, cette invention fournit des procédés et des compositions contenant une ou des métalloprotéase(s) neutre(s) obtenue(s) à partir du Bacillus sp.. Dans d'autres modes de réalisation préférés, la métalloprotéase neutre est obtenue à partir de B. amyloliquefaciens. Dans d'autres modes de réalisation préférés encore, la métalloprotéase neutre consiste en un variant de la métalloprotéase neutre de B. amyloliquefaciens ou en un homologue de la métalloprotéase neutre de B. amyloliquefaciens. Cette invention est particulièrement utilisée dans des applications telles que, entre autres, le nettoyage, le blanchiment et la désinfection.

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