Use of 13c-nmr to detect binding

Details Use of 13c-nmr to detect binding Use of 13c-nmr to detect binding

G - Physics

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G01N 24/08 (2006.01) G01N 33/50 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01) G01N 33/94 (2006.01) G01R 33/465 (2006.01)

Patent

CA 2365385

Methods of detecting binding of a putative ligand to a 13C-enriched target molecule, methods of screening for compounds which bind to a 13C-enriched target molecule, methods for calculating the dissociation constant of a ligand compound which binds to a 13C-enriched target molecule, and methods employed in the determination of the specific amino acids in a 13C-enriched target molecule affected by the binding of a ligand, as well as compounds identified by these screening methods, are provided herewith.

L'invention concerne des procédés pour détecter la liaison d'un ligand présumé à une molécule cible enrichie en <13>C, des procédés de criblage des composés se liant à une molécule cible enrichie en <13>C, des procédés servant au calcul de la constante de dissociation d'un composé ligand se liant à une molécule cible enrichie en <13>C, et des procédés servant à la détermination d'aminoacides spécifiques dans une molécule cible enrichie en <13>C affectée par la liaison d'un ligand. La présente invention porte également sur des composés identifiés par ces procédés de criblage.

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