Use of a chromatography substrate for reducing the amount of...

Details Use of a chromatography substrate for reducing the amount of... Use of a chromatography substrate for reducing the amount of...

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) A61K 38/36 (2006.01) A61P 7/04 (2006.01) C07K 1/18 (2006.01)

Patent

CA 2691604

The invention relates to the use of an ion-exchange chromatography substrate including a resin of the large-pore vinylic polymer type including DEAE groups and a buffer including trisodic citrate, sodium chloride, calcium chloride, glycine and lysine for reducing the amount of ADAMTS13 present in a solution derived from plasma containing a human von Willebrand factor.

L'invention consiste en une utilisation d'un support de chromatographie d'échanges d'ions comprenant une résine de type polymère vinylique à larges pores portant des groupements DEAE et d'un tampon comprenant du citrate trisodique, du chlorure de sodium, du chlorure de calcium, de la glycine et de la lysine pour réduire la quantité d'ADAMTS13 présente dans une solution dérivée du plasma contenant du facteur von Willebrand humain.

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