C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 5/076 (2010.01) C12Q 1/04 (2006.01) G01N 33/52 (2006.01)
Patent
CA 2561681
Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.
La présente invention concerne des mélanges de coloration comprenant des spermatozoïdes viables, une composition qui régule des réactions d'oxydation/réduction de manière intracellulaire ou extracellulaire et un colorant sélectif d'ADN. Les cellules contenues dans cette suspension tendent à posséder une plus grande capacité pour endurer les diverses étapes de processus habituellement associées au tri de cellules de sperme dans des populations à enrichissement par sexe, résultant en une composition après tri avec un nombre accru de spermes viables ou motiles. Cette invention concerne aussi des processus de coloration de cellules de sperme comprenant la formation d'un mélange colorant.
Anzar Muhammad
Crowley Kathleen S.
Graham Jeffrey A.
Ludwig Cindy L.
Inguran Llc
Mcfadden Fincham
Monsanto Technology Llc
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