Use of a fluorescent protein for detecting interaction...

G - Physics – 01 – N

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G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2295141

The invention concerns the use of a fluorescent protein selected in particular among the autofluorescent proteins for detecting the non-covalent interaction between a target protein marked by the fluorescent protein and one of its ligands marked by a marker consisting of: either a molecule capable of absorbing the light emitted by the fluorescent protein, or a fluorescent substance, said detection taking place by fluorescence energy transfer: between the fluorescent protein and said fluorescent substance, the fluorescent substance being such that it is excitable at the fluorescent protein emitting wavelength, or it emits at the fluorescent protein emitting wavelength; between the fluorescent protein and said molecule capable of absorbing the light emitted by the fluorescent protein.

L'invention concerne l'utilisation d'une protéine fluorescente choisie notamment parmi les protéines autofluorescentes, pour la détection de l'interaction non covalente entre une protéine cible marquée par la protéine fluorescente et l'un de ses ligands marquépar un marqueur constitué: soit par une molécule susceptible d'absorber la lumière émise par la protéine fluorescente, soit par une substance fluorescente, cette détection ayant lieu par transfert d'énergie de fluorescence: entre la protéine fluorescente et la susdite substance fluorescente, la substance fluorescente étant telle que soit elle est excitable à la longueur d'onde d'émission de la protéine fluorescente, soit elle émet à la longueur d'excitation de la protéine fluorescente, ou entre la protéine fluorescente et la susdite molécule susceptible d'absorber la lumière émise par la protéine fluorescente.

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