Use of a native epitope for selecting evolved binding...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/13 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01) C40B 30/04 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2396029

The invention provides a method for selecting at least one member from a library of proteinaceous molecules comprising providing at least one cell and/or a functional equivalent thererof, with at least part of said library under conditions that allow binding of any such member to an epitope in and/or on said cells and/or said functional equivalent thereof, removing unbound proteinaceous molecules and selecting said at least one member, wherein said library comprises at least one mutant of a proteinaceous molecule capable of binding to said epitope.

L'invention concerne un procédé de sélection d'au moins un élément, à partir d'une bibliothèque de molécules protéiniques, ce procédé consistant à mettre en contact au moins une cellule et/ou un équivalent fonctionnel de celle-ci, avec au moins une partie de cette bibliothèque, dans des conditions permettant la liaison dudit élément à un épitope, dans et/ou sur ces cellules et/ou l'équivalent fonctionnel de celles-ci, à éliminer les molécules protéiniques non liées et à choisir au moins ledit élément, la bibliothèque étant caractérisée en ce qu'elle comprend au moins un mutant d'une molécule protéinique capable de se lier audit épitope.

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