Use of a novel disintegrin metalloprotease, mutants,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/57 (2006.01) C12N 9/64 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2281085

This invention provides a method for identifying compounds capable of binding to the disintegrin protein, and determining the amount and affinity of a compound capable of binding to the disintegrin protein in a sample. This invention also provides a host cell comprising a recombinant expression vector to the disintegrin protein and a recombinant expression vector encoding to the disintegrin protein and the human disentegrin metalloprotease protein, fragment or mutant thereof, useful for these purposes. This invention also provides an in vivo or in vitro method for screening for osteoarthritis and other metalloprotease based diseases, capable of manufacture and use in a kit form.

La présente invention concerne un procédé permettant d'identifier des composés susceptibles de se fixer à la protéine disintégrine, et de déterminer la quantité et l'affinité d'un composé susceptible de se fixer à la protéine disintégrine dans un échantillon. L'invention concerne également une cellule hôte contenant un vecteur d'expression recombinant à la protéine disintégrine et un vecteur d'expression recombinant codant la protéine disintégrine, la protéine humaine métalloprotéase disintégrine, son mutant et son fragment. Ladite cellule est utilisée pour répondre aux objectifs de la présente invention. L'invention concerne enfin un procédé in vivo ou in vitro, qui permet de dépister l'ostéo-arthrite dégénérative et d'autres maladies provoquées par la métalloprotéase, et est susceptible d'être fabriqué et utilisé sous la forme d'un kit.

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