Use of a nucleotide sequence for enhancing protein synthesis...

Details Use of a nucleotide sequence for enhancing protein synthesis... Use of a nucleotide sequence for enhancing protein synthesis...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2397735

The present invention is related to the use of nucleotide sequences substantially similar to the cDNA sequence (SEQ ID NO:2:) obtainable from the leader sequence (SEQ ID NO:1:) of the Cocksfoot mottle virus (CfMV) which is capable of enhancing protein synthesis and expression of proteins, especially in plants such as cereals. Also disclosed is a method for producing potential enhancer elements by selecting 5'UTRs having a capacity of producing hairpin loop structures and preparing substantially similar nucleic acid sequences. In addition a method for enhancing the expression in plants as well as the properties characteristic for the nucleotide sequence which are responsible for the enhanced expression.

L'invention concerne l'utilisation de séquences nucléotidiques sensiblement similaires à la séquence d'ADNc (SEQ ID NO:2:), qui peuvent s'obtenir à partir de la séquence initiale (SEQ ID NO:1:) du virus de la mosaïque du dactyle et qui peuvent améliorer la synthèse de protéines et accroître l'expression de celles-ci, notamment dans des plantes telles que des céréales. L'invention concerne encore un procédé de production d'éléments d'accroissement du potentiel, consistant à choisir des 5'UTR capables de produire des structures de boucle en épingle à cheveux et de préparer des séquences d'acides nucléiques sensiblement similaires. En outre, l'invention concerne un procédé d'accroissement de l'expression dans des plantes, de même que les propriétés caractéristiques de la séquence nucléotidique responsable de cette expression accrue.

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