Use of a panel of heterokaryons to produce variants of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/15 (2006.01) C12N 15/04 (2006.01) C12N 15/10 (2006.01) C12P 21/02 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2260360

The present invention provides methods and compositions for producing and screening combinatorial libraries of multimeric proteins. The present invention relies on the use of filamentous fungal heterokaryons that are produced using two or more parent strains into which a population of DNA molecules encoding variants of a multimeric protein have been introduced.

La présente invention offre des méthodes et des compositions destinées à la production et au criblage de banques combinatoires de protéines multimères. La présente invention repose sur l'utilisation d'hétérocaryons fongiques filamenteux, produits au moyen de deux ou de plusieurs souches apparentées dans lesquelles on a introduite une population de molécules d'ADN codant des allèles d'une protéine multimère.

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