Use of a truncated elf-5a1 polynucleotide to induce...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61K 31/7105 (2006.01) A61K 39/235 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2735823

The present invention relates to the combinatorial use of an siRNA targeted against an endogenous gene to knock out or knock down expression of the endogenous gene in a host and a delivery of a polynucleotide encoding the gene in a delivery vehicle/expression vector to the host to provide expression in the host of the protein encoded by the polynucleotide. The present invention also relates to a truncated form of eIF-5A1 that is useful for inducing apoptosis and killing of cells, especially cancer cells in a mammal.

La présente invention porte sur l'utilisation combinatoire d'un ARNsi, ciblé contre un gène endogène pour activer ou inactiver l'expression du gène endogène dans un hôte, et de l'administration à l'hôte d'un polynucléotide codant pour le gène dans un véhicule d'administration/ vecteur d'expression afin d'obtenir l'expression dans l'hôte de la protéine codée par le polynucléotide. La présente invention porte également sur une forme tronquée de eIF-5Al qui est utile pour induire l'apoptose et tuer des cellules, notamment des cellules cancéreuses chez un mammifère.

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