Use of a tyrosine recombinase for genetic engineering

Details Use of a tyrosine recombinase for genetic engineering Use of a tyrosine recombinase for genetic engineering

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 9/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2450185

The present invention is directed to methods and compositions for TnpI- mediated genetic engineering using the Bacillus thurungiensis recombinase TnpI and the TnpI recombination substrates TRT or TRT', and variants thereof. In particular, the invention relates to TRT or TRT' sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT' sequences for TnpI-mediated genetic engineering.

La présente invention concerne des méthodes et des compositions destinées au génie génétique à médiation Tnpl, utilisant la recombinase TnpI du <i>Bacillus thurungiensis</i> et les substrats TnpI de recombinaison TRT ou TRT', et des variants correspondants. Notamment, cette invention a trait à des séquences TRT ou TRT' et des variants correspondants, des vecteurs, des cellules et des kits utilisés dans la recombinaison génétique à médiation Tnpl, ainsi que des méthodes d'utilisation de séquences TRT ou TRT' destinées au génie génétique à médiation Tnpl.

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