Use of .alpha.-1,4-glucan lyase for preparation of...

Details Use of .alpha.-1,4-glucan lyase for preparation of... Use of .alpha.-1,4-glucan lyase for preparation of...

C - Chemistry, Metallurgy

12

P

C12P 19/02 (2006.01) A23L 1/236 (2006.01) A23L 2/44 (2006.01) A23L 3/3562 (2006.01) A61K 31/7004 (2006.01) C07H 3/10 (2006.01) C12N 9/00 (2006.01) C12N 9/66 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2174116

A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an .alpha.-1,4-glucan with an .alpha.-1,4- glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the .alpha.-1,4-links, the .alpha.-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.

L'invention concerne un procédé de préparation du 1,5-D-anhydrofructose du sucre consistant à traiter un .alpha.-1,4-glucane avec une .alpha.-1,4-glucane lyase dans lequel l'enzyme est utilisée sous sa forme sensiblement pure. Dans un mode de réalisation préféré, si le glucane contient des liaisons autres que les liaisons .alpha.-1,4, ou des liaisons supplémentaires, on utilise l'.alpha.-1,4-glucane lyase en même temps qu'un réactif approprié capable de rompre ces autres liaisons.

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