Use of aspergillus sojae as host for recombinant protein...

Details Use of aspergillus sojae as host for recombinant protein... Use of aspergillus sojae as host for recombinant protein...

C - Chemistry, Metallurgy

12

N

C12N 15/80 (2006.01) C12N 1/14 (2006.01) C12N 9/58 (2006.01) C12N 15/10 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2378016

A recombinant Aspergillus sojae comprising an introduced acetamidase S (amdS) gene as selectable marker is disclosed. An Aspergillus sojae exhibiting growth with medium comprising uracil and fluoro-orotic acid, said Aspergillus sojae further not exhibiting growth on medium comprising uridine and fluoro-orotic acid i.e. said Aspergillus sojae exhibiting uracil auxotrophy, said Aspergillus sojae being unable to utilize uridine, said Aspergillus sojae being pyrG negative, said Aspergillus sojae exhibiting resistance to fluoro- orotic acid, said uracil auxotrophy and said fluoro-orotic acid resistance being relievable upon complementation with an active introduced pyrG gene, is described. The Aspergillus sojae further comprises a nucleic acid sequence encoding a phytase or a protein having phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said other heterologous proteins or polypeptides. Additional mutants exhibiting amended morphology are also disclosed. Methods of producing such expression hosts are described.

L'invention concerne un Aspergillus sojae) recombinant comprenant un gène S (amdS) acétamidase introduit en tant que marqueur sélectable. Elle concerne également un Aspergillus sojae qui exprime la croissance dans un milieu comprenant uracile et un acide fluoro-orotique, mais n'exprime pas la croissance dans un milieu comprenant uridine et un acide fluoro-orotique (c'est-à-dire, Aspergillus sojae exprime l'auxotrophie pour l'uracile, est incapable d'utiliser l'uridine, est pyrG négatif, est résistant à l'acide fluoro-orotique, peut être libéré après complémentation avec un gène pyrG) introduit). The Aspergillus sojae comprend en outre une séquence d'acide nucléique codant une phytase ou une protéine ayant une activité phytase ou toute autre protéine ou polypetide hétérologue, et peut être utilisé pour la production biotechnologique de cette phytase ou de ces autres protéines ou polypetides hétérologues. L'invention concerne en outre des mutants supplémentaires présentant une morphologie modifiée. Elle concerne enfin des méthodes de production de tels hôtes d'expression.

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