Use of autologous promoters to express gene products in...

C - Chemistry – Metallurgy – 12 – N

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167/135, 530/15.

C12N 15/31 (2006.01) A61K 39/10 (2006.01) C07K 14/235 (2006.01) C12N 1/21 (2006.01) C12N 15/74 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2040548

Autologous promoters are used to effect expression of gene products in pertussis strains. Hybrid pertussis genes are constructed comprising a structural pertussis gene fused at an ATG codon to a native but autologous pertussis promoter. B. pertussis, transformed by the hybrid gene by insertion into the chromosome of the organism by homologous recombination at specific loci, effects expression of the antigenic protein for which the structural gene codes at a production rate different from that achieved for the homologous gene. Specific strains and plasmids are descibed. The technique is applicable to a wide variety of gene products and organisms.

Des promoteurs autologues sont utilisés aux fins de la production de produits géniques par des souches de B. pertussis. Les gènes hybrides de B. pertussis construits comportent un gène structural de B. pertussis fusionné à un codon ATG à un promoteur indigène mais autologue de B. pertussis. La souche B. pertussis, transformée par le gène hybride par insertion dans le chromosome de l'organisme par recombinaison homologue à des locus précis, produit des protéines antigéniques codées par le gène structural à un taux de production différent de celui atteint dans le cas du gène homologue. Des souches et des plasmides spécifiques sont décrits. La technique s'applique à une grande variété de produits géniques et d'organismes.

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