Use of bacterial luciferase structural genes for cloning and...

Details Use of bacterial luciferase structural genes for cloning and... Use of bacterial luciferase structural genes for cloning and...

C - Chemistry, Metallurgy

12

Q

195/1.22, 195/1.

C12Q 1/68 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01) C12Q 1/06 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 1333776

A host microorganism is genetically and stably modified by the insertion into any of its non-essential chromosomal location of a non-homologous, recombinant foreign DNA fragment, maintaining an insertion of a luxAB gene of a selected bioluminescent bacterium such as V.harveyi, such that the expression of the luxAB genes causes the production of a luciferase enzyme which, in turn, catalyzes a light-emitting reaction in the presence of the appropriate substrate. X-ray film can be used to quantify the light being emitted from a microorganism through the use of plural droplets containing the same microorganism, each with a known and related cell (or plasmid) count.

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