C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/79 (2006.01) A01K 67/027 (2006.01) A61K 48/00 (2006.01) C12N 9/12 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2305815
The invention relates to the use of prokaryotic beta recombinase in eukaryotic cells, especially for transgenic work in eukaryotic cells. It also relates to the use of prokaryotic beta recombinase for site-specific intramolecular recombination between two six sites in eukaryotic cells. The use of the gene coding for beta recombinase for promoting the deletion of DNA sequences located between directly oriented six sites in mammalian cells and for catalysing site-specific resolution of DNA sequences in an extrachromosomal target introduced into an eukaryotic cell are also disclosed.
L'invention concerne l'utilisation d'une recombinase bêta procaryote dans des cellules eucaryotes, notamment pour le travail transgénique dans des cellules eucaryotes. L'invention concerne également l'utilisation de recombinase bêta eucaryote aux fins de recombinaison intramoléculaire spécifique au site entre deux sites six dans des cellules eucaryotes. L'invention concerne enfin l'utilisation du gène codant pour la recombinase bêta afin de favoriser la délétion de séquences ADN situées entre sites six à orientation directe dans des cellules de mammifères et de catalyser la résolution de séquences ADN spécifique au site dans une cible extrachromosomique introduite dans une cellule eucaryote.
Alonso Carlos Martinez
Alonso Juan C.
Bernad Antonio
Diaz Vicente
Rojo Fernando
Consejo Superior de Investigaciones Cientificas
Gowling Lafleur Henderson Llp
Pharmacia & Upjohn Ab
Pharmacia Spain S.a.
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