Use of blocking protein with high ph extraction in method to...

Details Use of blocking protein with high ph extraction in method to... Use of blocking protein with high ph extraction in method to...

G - Physics

01

N

G01N 33/569 (2006.01) C12N 1/06 (2006.01) G01N 1/28 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2077014

USE OF BLOCKING PROTEIN WITH HIGH pH EXTRACTION IN METHOD TO DETERMINE A MICROOGANISM ASSOCIATED WITH PERIODONTAL DISEASE AND KIT USEFUL THEREFOR Abstract of the Disclosure A method has been developed for determining microorganisms associated with periodontal diseases which is highly sensitive and shows very low background and cross-reactivity among various closely related antigens. Antigen is extracted at relatively high pH, and either before or immediately after extraction, the antigen-containing specimen is mixed with a blocking composition having at least about 0.2 weight percent of a non-immunoreactive blocking protein. The pH of the resulting mixture is kept high when contacted with the antibodies specific to the antigen of interest. The compositions and components needed for the assay can be supplied in a diagnostic test kit.

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