C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2621622
The present invention relates to the use of both RD9 and IS6110 as nucleic acid targets, for the specific and sensitive detection of a mycobacterium of the Mycobacterium tuberculosis complex (MtbC), and/or for the specific and sensitive discrimination of Mycobacterium tuberculosis and Mycobacterium canetti on one hand, from the other strains of said MtbC on the other hand. Advantageously, said MtbC detection and said species discrimination can be made in a single amplification run (multiplex PCR). The means of the invention are advantageous tools for the diagnosis of tuberculosis.
La présente invention concerne l'utilisation de RD9 et IS6110 en tant qu'acides nucléiques cibles, pour la détection spécifique et sensible d'une mycobactérie du complexe Mycobacterium tuberculosis (MtbC), et/ou pour la discrimination spécifique et sensible de Mycobacterium tuberculosis et Mycobacterium canetti d'une part, des autres souches dudit MtbC d'autre part. Avantageusement, la détection dudit MtbC et la discrimination desdites espèces peuvent être effectuées en un seul essai d'amplification (PCR multiplex). Les moyens de l'invention sont des outils avantageux pour le diagnostic de la tuberculose.
Clarebout Gervais
Savoye Chantal
Bio-Rad Innovations
Bio-Rad Pasteur
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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