C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07C 229/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2410388
The invention relates to the use of compositions for isolating and/or stabilising nucleic acids in or from micro-organisms such as prokaryots, fungi, protozoa or algae. The composition comprises a cationic compound of general formula Y+R1R2R3R4 X' as an essential constituent; wherein Y can represent nitrogen or phosphorus; R1, R2, R3 and R4 can independently represent an unbranched or branched C1-C20 alkyl radical and/or a C6-C20 aryl radical and a C6-C26 aralkyl radical; and X' can represent an anion pertaining to an inorganic or organic, monobasic or polybasic acid.
La présente invention concerne l'utilisation de compositions pour stabiliser des acides nucléiques dans des micro-organismes comme les procaryotes, les champignons, les protozoaires ou les algues- ou les isoler de ces acides nucléiques. La composition comprend comme composant essentiel un composé cationique de la formule générale Y?+¿R¿1?R¿2? R¿3?R¿4? X?-¿ où Y peut représenter de l'azote ou du phosphore, R¿1?, R¿2?, R¿3? et R¿4? peuvent indépendamment représenter un reste alkyle C¿1?-C¿20? non ramifié ou ramifié et/ou un reste alkyle C¿6?-C¿20?- ainsi qu'un reste aralkyle C¿6?-C¿26? et X?-¿ peut représenter un anion d'un acide monobasique ou polybasique inorganique ou organique.
Oelmuller Uwe
Wille Tanja
Fetherstonhaugh & Co.
Qiagen Gmbh
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