Use of conserved oligonucleotide primers to amplify human...

C - Chemistry – Metallurgy – 07 – H

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195/1.12, 150/8.

C07H 21/04 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2029126

31 ABSTRACT OF THE DISCLOSURE Two oligonucleotides are provided which function as amplifying primers when used in tandem in the polymerase chain reaction with HPV DNA. The primers are of the for- mulae: IU: 5 -TIIN1IN1IIN2TAAAACGAAAGT-3 and IWDO: 5 -N1TCN1N3AIGCCCAN2TGIACCAT-3 wherein N1 is A or G, N2 is C or T and N3 is A or T.

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