Use of cyclodextrins to improve the specificity, sensitivity...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2746021

The invention is directed to methods for in vitro DNA synthesis catalysed by a DNA polymerase using cyclodex-trins. The invention also relates to methods, compositions and kits comprising cyclodextrins for the amplification of a nucleic acid. The use of cyclodextrins improves the specificity, sensibility and/or yield of the amplification reaction. The invention is re-lated more particularly to kits, compositions and methods for carrying out PCR reactions comprising a cyclodextrin.

La présente invention concerne des procédés de synthèse d'ADN in vitro catalysée par une ADN polymérase et utilisant des cyclodextrines. Linvention concerne également des procédés, des compositions et des kits comprenant des cyclodextrines destinés à l'amplification d'un acide nucléique. L'utilisation des cyclodextrines améliore la spécificité, la sensibilité et/ou le rendement de la réaction d'amplification. L'invention concerne plus particulièrement des kits, des compositions et des procédés destinés à mettre en uvre des réactions PCR comprenant une cyclodextrine.

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