Use of defibrinated blood for manufacture of a...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/078 (2010.01) A61K 35/14 (2006.01) A61K 35/18 (2006.01) A61K 38/42 (2006.01) C07K 14/805 (2006.01)

Patent

CA 2439123

Red blood cells are purified by defibrinating whole blood and then filtering the defibrinated whole blood, whereby at least a portion of a plasma component is separated from the red blood cells to form a suspension of red blood cells, thereby purifying the red blood cells. Whole blood is defibrinated by, for example, using a chemical coagulating agent or mechanical agitation. Separation of the plasma component from red blood cells can be completed by, for example, diafiltration. The suspension of red blood cells can then be employed to produce a hemoglobin-based oxygen carrier.

Procédé de purification de globules rouges, qui comporte les étapes consistant à défibriner du sang entier et à filtrer ensuite le sang entier défibriné, ce qui permet de séparer au moins une partie du constituant plasmatique des globules rouges et de former une suspension de globules rouges. Le sang entier est défibriné, par exemple, au moyen d'un agent de coagulation chimique ou par agitation mécanique. La séparation du constituant plasmatique des globules rouges peut être mise en oeuvre, par exemple, par diafiltration. La suspension de globules rouges peut ensuite servir à produire un substitut sanguin à base d'hémoglobine.

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