Use of disk surface for electroporation of adherent cells

Details Use of disk surface for electroporation of adherent cells Use of disk surface for electroporation of adherent cells

C - Chemistry, Metallurgy

12

M

C12M 1/42 (2006.01) C12N 15/87 (2006.01) G01N 15/00 (2006.01)

Patent

CA 2721719

Adherent cells on the surface of a disk are transfected by electroporation between coaxial circular cylinders with electrodes on the opposing surfaces on either side of the annular space between the cylinders by placing a buffer solution containing the transfecting species in the annular space over the cell and applying an electric potential between the electrodes.

Des cellules adhérentes à la surface dun disque sont transfectées par électroporation entre des cylindres circulaires coaxiaux, avec des électrodes sur les surfaces opposées de chaque côté de lespace annulaire entre les cylindres, par mise en place dune solution tampon contenant les espèces transfectantes dans lespace annulaire au-dessus de la cellule et par application dun potentiel électrique entre les électrodes.

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