Use of dna-dependent rna polymerase transcripts as reporter...

Details Use of dna-dependent rna polymerase transcripts as reporter... Use of dna-dependent rna polymerase transcripts as reporter...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 19/06 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01) G01N 33/532 (2006.01)

Patent

CA 2070632

2070632 9110746 PCTABS00006 A polydeoxynucleotide construct is disclosed for use, in conjunction with a DNA-dependent RNA polymerase, as a signal amplifier in nucleic acid hybridization assays. The construct contains a recognition sequence for a target oligonucleotide, a promoter sequence for a DNA-dependent RNA polymerase, and a polymerase template. A method of use for this construct in hybridization assays is also disclosed. The method involves formation of a hybridization complex comprising the construct and the target sequence; addition of a polymerase which is specific for the promoter in the construct; and quantification of the resulting RNA transcripts.

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