Use of electrochemical detection of redox species in a...

Details Use of electrochemical detection of redox species in a... Use of electrochemical detection of redox species in a...

C - Chemistry, Metallurgy

12

Q

324/23

C12Q 1/00 (2006.01) C12M 1/40 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1239191

ABSTRACT USE OF ELECTROCHEMICAL DETECTION OF REDOX SPECIES IN A MEDIUM IN COMBINATION WITH DEGRADABLE SUBSTRATES The invention concerns the use of an electrochemical detection of redox species (MR) in a medium to determine the occurence of a condition under which such species are produced or released, wherein such production or release is enabled by the digestion or disruption a macromolecular species (An). The invention provides a method of assay for a polymer, for a depolymerase enzyme, and for a labelled specific binding agent, in which a polymer (An) is labelled with a component of a mediator/enzyme/substrate system which remains inactive until released into solution. Electrodes in the medium determine the occurence of A condition under which redox species are produced by the mediator/enzyme/substrate system or released into solution. Where the mediator activity of, for example ferrocene to glucose oxidase, is used an amplification step provided. Refer to figure 1.

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