Use of excipients to increase dna uptake by swine muscle cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) A61K 47/00 (2006.01) A61K 48/00 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2499533

The present invention provides novel excipient formulations for delivering nucleic acids, particularly naked DNA, across cell membranes in vivo. The present invention also provides penetration enhancers which can be used to enhance delivery and uptake by cells of a wide variety of nucleic acids particularly therapeutic agents or DNA molecule containing a gene encoding a protein which can benefit normal animals, especially in the application of gene therapy and augmenting viability or survival rate of newborn farm animals.

L'invention concerne des formulations d'excipients destinées à délivrer des acides nucléiques, en particulier de l'ADN nu, <i>in vivo</i> à travers des membranes cellulaires. Elle concerne aussi des agents améliorant la pénétration qui peuvent être utilisés afin d'augmenter la quantité délivrée et prise par les cellules d'une grande variété d'acides nucléiques, en particulier d'agents thérapeutiques ou de molécules d'ADN contenant un gène codant pour une protéine bénéfique aux animaux normaux, plus spécialement dans un but de thérapie génique et d'augmentation de la viabilité ou du taux de survie de jeunes animaux d'élevage.

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