Use of fkbp chaperones as expression tool

Details Use of fkbp chaperones as expression tool Use of fkbp chaperones as expression tool

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A61K 38/00 (2006.01) A61K 47/48 (2006.01) C07K 14/16 (2006.01) C12N 5/10 (2006.01) C12N 9/90 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2449747

The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor, methods of recombinant protein expression, the recombinant polypeptides thus obtained as well as to the use of such polypeptides.

L'invention concerne le clonage et l'expression d'une protéine exogène ou de polypeptides dans des bactéries telles que l'Escherichia coli. L'invention concerne notamment des instruments d'expression comprenant une peptidyle propyle isomérase de type FKBP sélectionnée dans le groupe comprenant le facteur FkpA, SlyD et le facteur déclic. L'invention concerne en outre des méthodes d'expression de protéine recombinée, les polypeptides recombinés ainsi obtenus, de même que l'utilisation desdits polypeptides.

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