Use of flow-cytometric analysis to optimize cell banking...

Details Use of flow-cytometric analysis to optimize cell banking... Use of flow-cytometric analysis to optimize cell banking...

G - Physics

01

N

G01N 33/569 (2006.01) A01N 1/02 (2006.01)

Patent

CA 2578400

Production of biopharmaceuticals from CHO cells requires generation of master- , working- and post-production cell banks of high quality, partly under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to the desire to establish production processes that are completely devoid of serum or even any animal components and to ensure robust thaw performance for reliable production. Here we describe a novel strategy employing flow cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of CHO cell banks. Our data show that this method enables evaluation of a cryopreservation procedure just 6h after thaw.

La préparation de produits biopharmaceutiques à partir de cellules CHO nécessite de générer des bibliothèques cellulaires avant, pendant et après la production de qualité élevée, partiellement dans des conditions GMP. Une stratégie optimiser de cryopréservation nécessite d'être appliquée pour chaque nouvelle lignée cellulaire de production, en particulier dans le but de mettre en oeuvre des processus de production totalement exempts de sérum ou d'autres constituants animaux et d'assurer une décongélation efficace. L'invention concerne une nouvelle stratégie mettant en application une analyse cytométrique de flux (FC) de cellules marquées par Annexine V afin d'effectuer une caractérisation productive de bibliothèque cellulaire de cellules CHO. Cette méthode permet d'évaluer une procédure de cryopréservation après une décongélation de 6 heures seulement.

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