Use of haploid genomes for genetic diagnosis, modification...

A - Human Necessities – 01 – N

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A01N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 15/87 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2388510

Methods for propagating haploid genomes of male or female origina and genetic screening and modification thereof are provided. These haploid genomes may be used to produce haploid embryos, and embryonic stem-like cells and differentiated cells. Also, these haploid genomes and cells containing, may be used as nuclear transfer donors to produce diploid nuclear transfer units. These diploid NT units e.g., human NT units, may be used to obtain pluripotent cells and differentiated cells and tissues.

La présente invention concerne des procédés permettant de propager des génomes haploïdes d'origine mâle ou femelle et de criblage et modification génétique de ceux-ci. Ces génomes haploïdes conviennent à la production d'embryons haploïdes, ainsi que de cellules embryonnaires de type souche et de cellules différenciées. Il en résulte que ces génomes haploïdes et les cellules les contenant conviennent comme donneurs de transfert nucléaires pour la production d'unité de transfert nucléaires diploïdes. Ces unités de transfert nucléaires diploïdes, et notamment d'origine humaine, conviennent à l'obtention de cellules totipotentes, et de cellules et tissus différenciés.

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