Use of helper-dependent adenoviral vectors of alternative...

Details Use of helper-dependent adenoviral vectors of alternative... Use of helper-dependent adenoviral vectors of alternative...

C - Chemistry, Metallurgy

12

N

C12N 15/861 (2006.01) A61K 48/00 (2006.01) C12N 15/63 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2367390

This invention responds to a long felt need, by providing in one embodiment, a helper virus based on the Ad2 serotype for use in the Cre/loxP system for the generation of Ad vectors deleted of all Ad protein coding sequences. Using this and helper virus based on Ad5, genetically identical hdAd that differ only in the virion protein components, which are derived from the helper virus, were produced. The vectors have identical expression characteristics in vitro, regardless of the serotype, and the sequential use of hdAd of different serotypes allows for successful repeat vector administration in vivo.

Dans un mode de réalisation, l'invention concerne un virus assistant basé sur le sérotype Ad2, destiné à être utilisé dans le système CreloxP permettant la génération de vecteurs Ad ne contenant plus aucune séquence codante pour les protéines Ad. Des hdAd génétiquement identiques ont pu être produits, à l'aide de ce virus assistant et d'un virus assistant basé sur Ad5, se distinguant seulement par les composants de la protéine vif, dérivés du virus assistant. Les vecteurs possèdent des caractéristiques d'expression identiques in vitro, indépendamment du sérotype, et l'utilisation séquentielle de hdAd de différents sérotypes permet de répéter avec succès l'administration du vecteur in vivo.

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